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For the validation cohort (2021), a similar protocol than above was used in 96 well plates with the following modifications: Digestion was made with 0.25 g of trypsin only, acidification was performed with formic acid 1% (FA)

Edetate Calcium Disodium (usp) 124

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As for the idea that GH can have an impact on athletic performance or aging, there is a lack of evidence that it can do anything of the sort